p ril 2023 human monocyte line thp1 cells  (ATCC)

Journal: eLife

Article Title: PI3Kα inhibition blocks osteochondroprogenitor specification and the hyper-inflammatory response to prevent heterotopic ossification

doi: 10.7554/eLife.91779

Figure Lengend Snippet: Cells were cultured for 6 days and in control conditions, with BMP6 (2 nM) and/or BYL719 (2 or 10 µM). Areas under the proliferation curves were compared by one-way ANOVA with Dunnett’s multiple comparisons test against the control. (*) refers to significant differences between control and single treatments (BMP6 or BYL719). (#) refers to significant differences between control and combined treatments. Data are shown as mean ± SD ( n = 4 per group). ** or p < 0.01, *** or ### p < 0.001. ( D ) Migration assay of THP1 cells with control conditions, or with FBS or BMP6 as chemotactic agents with or without BYL719 treatment. Data are shown as mean ± SD ( n = 4 per group). ***p < 0.001, two-way ANOVA with Tukey’s multiple comparisons test. Gene expression assays of THP1 ( E ), RAW264.7 ( F ), and HMC-1 ( G ) cells. Cells were treated for 48 hr with BYL719 (2 µM) and/or BMP6 (2 nM). Data are shown as mean ± SD ( n = 4 per group). *p < 0.05, **p < 0.01, ***p < 0.001, one-way ANOVA with Dunnett’s multiple comparisons test, significance shown between control group and other groups.

Article Snippet: The human monocyte cell line THP1 (ATCC) was cultured in RPMI-1640 supplemented with 10% FBS, 2 mM glutamine, 1 mM sodium pyruvate, 100 U/ml P/S, 0.1 mM NEAA, 10 mM HEPES, and 50 μM 2-mercaptoethanol.

Techniques: Cell Culture, Control, Migration, Gene Expression

Journal: Frontiers in Oncology

Article Title: Interrogation of macrophage-related prognostic signatures reveals a potential immune-mediated therapy strategy by histone deacetylase inhibition in glioma

doi: 10.3389/fonc.2025.1554845

Figure Lengend Snippet: Prediction and validation of the potential anticancer drugs. (A) Correlation between CD163 expression and risk score in the TCGA database (p <0.0001). (B) Differences in mRNA expression of various prognostic genes and CD163 in the indirect co-culture model (*p <0.05; * *p <0.01; * * *p <0.001; * * * *p <0.0001). (C) Expression of CD163 and risk score in the indirect co-culture model. (D) Potential anticancer drugs were predicted using signature genes and a risk score. (E) The U87MG (RP) was cocultured with the PMA-induced adherent THP1 (GP), and the treatment groups were then treated with Vorinostat. (F) Analysis of growth of U87MG and U87MG cocultured with PMA-induced adherent THP1 before and after Vorinostat treatment. (G) Expression of macrophage-related immune checkpoints of THP1 before and after the treatment with Vorinostat in the U87MG-THP1 indirect co-culture model (*p <0.05; * *p <0.01; * * *p <0.001; * * * *p <0.0001). (H) The expression of Cd163 in RAW264.7 cells at the protein level, with GL261 conditioned medium and Vorinostat as variables.

Article Snippet: Human glioma cell lines A172 and U87MG, human monocytic leukemia cell line THP1, mouse macrophages cell line RAW264.7 were obtained from American Type Culture Collection (ATCC) (Manassas, VA, USA).

Techniques: Biomarker Discovery, Expressing, Co-Culture Assay